"
Team:Gifu/Notebook
From 2014.igem.org
Calender
Click on a date to see our research activities on that day.
| August | September | |||||||||||||||
| Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
| 11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
| 18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
| 25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 | > | |||||||
August
August 11th
Restriction Enzyme Digestion
| sample | restriction enzymes |
|---|---|
| His-RFP with stop codon | SpeI,EcoRI | 5'-intron M | SpeI,EcoRI |
| Lac-3'intron-RBS | SpeI,EcoRI |
| His-RFP without stop codon | XbaI,EcoRI |
| RBS-Amp | SpeI,EcoRI |
Ligation
- EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
- EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI
Transformation
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm
- His-RFP(without stop codon)-Amp
Densitometry
| sample | concentration(ng/µL) |
|---|---|
| His-RFP(with stop codon) EcoRI-SpeI | 162.6 |
| 5'intron M EcoRI-SpeI | 45.4 |
| Lac-3'intron-RBS EcoRI-SpeI | 120.9 |
| His-RFP(without stop codon) EcoRI-SpeI | 160.0 |
| His-RFP(without stop codon)-5'intron-DT-Cm XbaI-EcoRI | 174.0 |
| Amp EcoRI-SpeI | 101.8 |
August 12th
Colony PCR
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm
- His-RFP(without stop codon)-Amp
Electrophoresis
- His-RFP(with stop codon)-DT
- His-RFP
- 5'intron M-DT
- 5'intron M
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[1]
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[2]
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[3]
- His-RFP(without stop codon)-5'intron-DT
- His-RFP(without stop codon)
Liquid Culture
- His-RFP(with stop codon)-DT
- 5'intron M-DT
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[1]
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[3]
- His-RFP(without stop codon)
August 13th
Miniprep
PCR
- By use of KAPA
Electrophoresis
Restriction Enzyme Digestion
Ligation
Transformation
August 16th
Liquid Culture
August 18th
Miniprep
Colony PCR
Densitometry
Ligation
Transformation
Analysis of DNA sequences
August 19th
Colony PCR
Electrophoresis
Shaking Culture
August 20th
Colony PCR
Same as August 19th Colony PCR.
