Team:Evry/Notebook/Transformation/08-25-2014

From 2014.igem.org

Revision as of 14:21, 29 August 2014 by NoémieBC (Talk | contribs)

Picture

Amplification of pRhokHI-2
We re-tried to transform E.Coli with pRhokHI-2 to amplify it (LIEN PROTO). This time, after a liquid culture of only one hours cells were plated on LB 1X + Kan (1:1000) and let in incubator overnight.

Transformation of Pseudovribrio with pBBR1MCS
After made a MiniPrep purification of pBBR1MCS (LIEN PROTO), electrocompetent Pseudovibrio cells were tranformed with the plasmid by electroporation (LIEN PROTO), then they were incubated for three hours and showed on MB 1X+Cam (1:1000) plates. Plates are let in incubator overnight.

Aug 25

Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Transformation/08-25-2014"