Team:Evry/Notebook/Transformation/08-28-2014

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Picture

Transformation of Pseudovribrio with pRhokHI-2
After incubation overnight we can see some colonies of E.Coli Bl21 transformed on LB+Cam 1:1000 and on LB+Kan 1:1000. Our Bl21 which had not been transformed doesn't growth on these media but both grow on LB only. We had well success to tranform E.Coli and amplifly the plasmid.

Transfo2


We tranfered two colonies into liquid MB+Kan(1:1000) and let them in incubator overnight to have a pre-culture to the plasmid purification.

Aug 28

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