Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014

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Picture

Amplification of plasmids pBBR1MCS and pRhokHI-2
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)

plasmidS

Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate).
After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight.

Aug 23

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