Team:NCTU Formosa/project

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Project

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Contents

Overview

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Figure 1. We attempt to construct a regulated system that can express different genes under different conditions.


PBAN

Introduction

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Figure 2. Phycocyanobilin is the part of photoreceptor that responds to light, and it is not naturally produced in E.coli,so we introduced two phycocyanobilin-biosynthesis genes (ho1 and pcya) from Synechocystis that convert heme into phycocyanobilin.

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Figure 3. The light receptor cph8 is composed of cph1(pink) and envZ-ompR(maroon).


Design of Red promoter

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Reference
  1. part BBa_I15008;MIT Registry of Standard Biological Parts
  2. part BBa_I15009;MIT Registry of Standard Biological Parts
  3. Levskaya, A. et al .(2005). Engineering Escherichia coli to see light. Nature, 438(7067), 442.
  4. Kehoe DM, Grossman AR (1996) Similarity of a chromatic adaptation sensor to phytochrome and ethylene receptors. Science 273(5280):1409–1412
  5. Yeh KC, Wu SH, Murphy JT, Lagarias JC (1997) A cyanobacterial phytochrome two-component light sensory system. cience 277 (5331):1505–1508
  6. Dutta R, Qin L, Inouye M (1999) Histidine kinases: diversity of domain organization. Mol Microbiol 34(4):633–640
  7. Forst SA, Roberts DL (1994) Signal transduction by the EnvZ–OmpR phosphotransfer system in bacteria. Res Microbiol 45(5–6):363–373
  8. Thomas Drepper, Ulrich Krauss,Sonja Meyer zu Berstenhorst, Jörg Pietruszka, Karl-Erich Jaeger.(2011).Lights on and action! Controlling microbial gene expression by light. Appl Microbiol Biotechnol, 90:23–40 DOI:10.1007/s00253-011-3141-6

Design

Introduction

Mechanism

Design

Reference
  1. Torsten Waldminghaus, Nadja Heidrich, Sabine Brantl and Franz Narberhaus .(2007). FourU: a novel type of RNA thermometer in Salmonella . Molecular Microbiology , 65(2): 413–424 DOI:10.1111/j.1365-2958.2007.05794.x
  2. part BBa_K115002;TUDelft Registry of Standard Biological Parts

Device

Introduction

Mechanism

Design

Reference
  1. Xu, S.; Montgomery, M.; Kostas, S.; Driver, S.; Mello, C. (1998). "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans". Nature 391 (6669): 806–811 DOI:10.1038/35888
  2. Jörg Vogel , Ben F. Luisi.(2011). Hfq and its constellation of RNA. Nature Reviews Microbiology, 9:578-589
  3. E.K. Jocelyn, S.G. Elliott , T.K. Stephen, "Lewin's Genes X.-10th ed.", Jones & Bartlett, Sudbury, MA, 2011.
  4. Karen M. Wassarman.(2002). "Small RNAs in Bacteria: Diverse Regulators of Gene Expression in Response to Environmental Changes". Cell, 109:141–144
  5. Hongmarn Park, Geunu Bak, Sun Chang Kim & Younghoon Lee.(2013). "Exploring sRNA-mediated gene silencing mechanisms using artificial small RNAs derived from a natural RNA scaffold in Escherichia coli ". Nucleic Acids Research,Vol. 41, No. 6, 3787-3804 DOI:10.1093
  6. Vandana Sharma, Asami Yamamura & Yohei Yokobayashi.(2011). "Engineering Artificial Small RNAs for Conditional Gene Silencing in E. coli". ACS Synthetic Biology


Cover image credit: DVQ

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