Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014

From 2014.igem.org

Revision as of 11:51, 28 August 2014 by NoémieBC (Talk | contribs)

Picture

Amplification of plasmids pBBR1MCS and phokHI-2
Two plasmids pBBR1MCS and phokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)

pBBR1MCS phokH1-2

Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate). After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight.

Aug 23

Retrieved from "http://2014.igem.org/Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014"