Team:Gifu/Notebook
From 2014.igem.org
Calender
Click on a date to see our research activities on that day.
August | September | |||||||||||||||
Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 |
August
August 11th
Restriction Digests
object | restriction enzymes |
---|---|
His-RFP with stop codon | SpeI,EcoRI | 5'-intron M | SpeI,EcoRI |
Lac-3'intron-RBS | SpeI,EcoRI |
His-RFP without stop codon | XbaI,EcoRI |
RBS-Amp | SpeI,EcoRI |
Ligation
- //EcoRI-His-RFP(with stop codon)-SpeI// + //XbaI-DT-Cm-EcoRI//
- //EcoRI-5'intron M-SpeI// + //XbaI-DT-Cm-EcoRI//
- //EcoRI-Lac-3'intron-RBS-SpeI// + //XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI//
- //EcoRI-His-RFP(without stop codon)-SpeI// + //SpeI-Amp-EcoRI//
Transformation
August 12
sasisuseso
August 13
sasisuseso