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August										September
Mon	Tue	Wed	Thr	Fri	Sat	Sun				Mon	Tue	Wed	Thr	Fri	Sat	Sun
				1	2	3				1	2	3	4	5	6	7
4	5	6	7	8	9	10				8	9	10	11	12	13	14
11	12	13	14	15	16	17				15	16	17	18	19	20	21
18	19	20	21	22	23	24				22	23	24	25	26	27	28
25	26	27	28	29	30	31				29	30

August

August 11th

Restriction Digests

object	restriction enzymes
His-RFP with stop codon	SpeI,EcoRI
5'-intron M	SpeI,EcoRI
Lac-3'intron-RBS	SpeI,EcoRI
His-RFP without stop codon	XbaI,EcoRI
RBS-Amp	SpeI,EcoRI

Ligation

• {EcoRI-His-RFP(with stop codon)-SpeI} + {XbaI-DT-Cm-EcoRI}
• {EcoRI-5'intron M-SpeI} + /XbaI-DT-Cm-EcoRI}
• {EcoRI-Lac-3'intron-RBS-SpeI} + {XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI}
• {EcoRI-His-RFP(without stop codon)-SpeI} + {SpeI-Amp-EcoRI}

Transformation

back to calender

August 12

sasisuseso

back to calender

August 13

sasisuseso

back to calender