Today is a short day. Yesterday, we picked 2 colonies from each failed clone plates and inoculate them; today, we minipreped and test digested them.

We are sending the gRNA from the third batch and some other reporter vectors for sequencing.

Yesterday, we digested more RBS-Carb; today, we ran it on gel and gel purified it. The concentration at the end was good.

Our clones on the plates transformed yesterday were good: many colonies on the plates and none on the negative.

Orientation! School is starting next week! (25/8) >.<

• Miniprep ctxA, ctxB.
• Test digest and ran gel for ctxA, ctxB.
• Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
• Prepared for sequencing: diluted to the correct volume and concentration.
• Purified RBS (digested overnight yesterday).
• Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).

RBS-Carb Gel was good.

Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.