Team:UT-Dallas/Notebook/8-18
From 2014.igem.org
Today is a long day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone in the weekend. We are digesting second batch of reporter vectors, ligate and transform them.
Hopefully tomorrow there will be colonies on our plates.
We also digest RBS-Carb to get more Carb back bone for later use.
We started the third batch last week with some gel digest testing of gRNA. Only one (tcpF) failed. We are building our reporter vectors today from PCR products.