Team:HIT-Harbin/Judging

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Parts

Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote

CODON OPTIMIZATION FOR HRPS AND HRPR GENE

Figure1: Codon Adaptation of hrpS gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17%

Figure2: Codon Adaptation of hrpS gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60%

Figure3: Codon Adaptation of hrpR gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70

Figure4: Codon Adaptation of hrpR gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69%

reference

[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33
NameTypeDescriptionDesignerLength
A  BBa_K1014000CodingHrpS GeneXiangmiao Zeng987
A BBa_K1014001CodingHrpR GeneXiangmiao Zeng948
A BBa_K1014002RegulatoryPromoter hrpLXiangmiao Zeng210
A  BBa_K1014004CompositeHrpR Protein Generator(W)Xiangmiao Zeng1168
A  BBa_K1014006CompositeHrpS Protein Generator(M)Xiangmiao Zeng1205
A  BBa_K1014007CompositeHrpS Protein Generator(W)Xiangmiao Zeng1206
A  BBa_K1014009CompositetetR Protein Generator(M)Xiangmiao Zeng1059
A  BBa_K1014010CompositetetR Protein Generator(W)Xiangmiao Zeng1060
A  BBa_K1014012CompositeRFP Protein Generator(M)Xiangmiao Zeng1080
A  BBa_K1014019CompositeHrpR Protein Generator(M)Xiangmiao Zeng1168
A  BBa_K1014998CompositeHrpS Protein GeneratorXiangmiao Zeng54
   BBa_K1014003CompositeHrpR Protein Generator(S)Xiangmiao Zeng1169
  BBa_K1014005CompositeHrpS Protein Generator(S)Xiangmiao Zeng1207
   BBa_K1014008CompositetetR Protein Generator(S)Xiangmiao Zeng1061
   BBa_K1014011CompositeRFP Protein Generator(S)Xiangmiao Zeng1082
   BBa_K1014013CompositeRFP Protein Generator(W)Xiangmiao Zeng1081
  BBa_K1014014CompositehrpL AND Gate deviceXiangmiao Zeng3474
   BBa_K1014015CompositeBiological proportional operational Mu-circuit(1)Xiangmiao Zeng4540
   BBa_K1014016CompositeHrpS Protein GeneratorXiangmiao Zeng1207
U  BBa_K1014997CompositetetR Protein Generator(S)Xiangmiao Zeng1324

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