WELCOME TO iGEM 2014!!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!

Click here to edit this page!

Plasmid transfer

We are working on building different kinds of plasmid transfer system. The Special plasmid that we constructed is transferred to the different cell by different ways. Here are two project for plasmid transfer.

For prokaryote:

Previous studies have shown that DNA transfer by bacterial conjugation requires a mating pair formation (Mpf) system that specifies functions for establishing the physical contact between the donor and the recipient cell and for DNA transport across membranes. During bacterial conjugation, the single-stranded DNA molecule is transferred through the cell envelopes of the donor and the recipient cell. For example the IncPa plasmid RP4, a thorough sequence analysis of the gene products of the transfer regions Tra1 and Tra2 revealed typical features of mainly inner membrane proteins, both of which contribute to Mpf.

Our project aims to construct a system of double plasmids, one is a mini plasmid carrying the oriT sequence and the other is a plasmid carrying the transfer regions of the broad-host-range IncP plasmid RP4, moreover, the two plasmids we construct are compatible. In the transfer system, the large one doesn’t carry oriT region while it carry the transfer regions tra and trb, due to lacking the sequence of oriT, the large plasmid can’t transfer between cells, thus the recipient strain don’t have the ability of conjugation. In the meantime, the other plasmid carrying oriT can transfer under the induction of the tra1 and tra2.

For eukaryote:

Fish diseases cause billion loss in the aquaculture filed every year. Traditional therapeutic method may do harm to the fishes and environment. However, using plasmid that contains the antigen genes as a new kind of vaccine can cause the immune reaction.

To achieve that goal, we will construct the recombinant pet32a (+) - TAT :: H4 plasmid, and transform into competent cells BL21 (DE3) in order to produce TAT :: H4, the products were purified and finally wewant to gain the fusion protein. Meanwhile for transfection of fish cells, we are going to construct carrying EGFP and CMV promoter plasmid pcDNA3.1 (+) - EGFP plasmid for detecting the expression and effect of transfection in eukaryotic cells.

Requirements

Please be sure to keep these links, your audience will want to find your:

• Home
• Team
• Official Team Profile
• Project
• Parts
• Modeling
• Notebook
• Safety
• Attributions

There are a few wiki requirements teams must follow:

• All pages, images and files must be hosted on the 2014.igem.org server.
• All pages must be created under the team’s name space.
• As part of your documentation, keep the links from the menu to the left.
• Do not use flash in wiki code.
• The iGEM logo should be placed on the upper part of every page and should link to 2014.igem.org.

Visit the Wiki How To page for a complete list of requirements, tips and other useful information.

Tips

We are currently working on providing teams with some easy to use design templates.
In the meantime you can also view other team wikis for inspiration! Here are some very good examples

• 2013 SDU Denmark
• 2013 SYSU China
• 2013 Shenxhen BGIG ATCG
• 2013 Colombia Unianades
• 2013 Lethbridge

For a full wiki list, you can visit iGEM 2013 web sites and iGEM 2012 web sites lists.

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

• State your accomplishments! Tell people what you have achieved from the start.
• Be clear about what you are doing and what you plan to do.
• You have a global audience! Consider the different backgrounds that your users come from.
• Make sure information is easy to find; nothing should be more than 3 clicks away.
• Avoid using very small fonts and low contrast colors; information should be easy to read.
• Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2013 calendar
• Have lots of fun!