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Team:Gothenburg/Calendar
From 2014.igem.org
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The team received mandatory safety training and general lab routine information.
A really cool video about Chalmers' chemical safety routine (recorded at our very own lab!) can be seen in http://www.chalmers.se/insidan/EN/about-chalmers/environmental-work/policies/chemical-safety-routine |
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As a part of the mandatory training required by the SysBio group, the team received instructions on how to operate the centrifuges
and autoclaves of the labs.
We also worked on our risk declaration; a mandatory document containing our experiments descriptions and information about the chemicals we are going to use, their handling, storage and associated risk, as well as waste handling. |
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Our Risk Declaration was approved by the lab manager and our supervisors. We also sign a safety certificate stating that we are aware of the safety instructions and with that, completed the mandatory "check-in list for new incomers" of the lab. |
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On the first day of laboration, we prepared LB, YPD and SC-Ura/His/Trp-Leu and YPD media.
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Succesfully PCR amplified five out of the seven parts from yeast genome via a Phusion High-Fidelity DNA Polymerase.
The PCR program used was:
A diagnostic gel was performed and the result was as follows:
[[File: Bio-Rad 2014-06-25 15hr 34min.jpg|frameless|300px]] The purification of the succesfully amplified parts was done using a PCR Purification Kit from GenJet following the instructions
of the manufacture's manual but with no addition of isopropanol. |
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