Requirements

Target

Firstly, we aim to introduce the new expression host Aspergillus Niger, which is a significant industrial fermentation microbial to iGEM. It's well known for efficient capacity of secreting proteins and recognized as the safe strain for heterologous protein production. Secondly, we want to exploit the visual gene replacement system. We design to utilize the fluorescent proteins to realize the indication of the target gene replacement and make the process continuable. The fluorescent proteins work as selective markers to help us pick out our target transformants without complex molecular detections. Thirdly, we import carotenoid metabolic pathway where all the enzymes are connected by the linker peptides to our vector, so that we can examine the feasibility of our system. Now we are still constructing our expression vectors. We have ligated HPH, GFP with linker and one of GlaA3s. Now we are trying to ligate GlaA5 and another GlaA3. When we finish our expression vector, we will transform the well-constructed plasmid into the Agrobacterium and use the Agrobacterium to infect the Aspergillus niger to carry our plasmid in the Aspergillus niger.

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