Team:UESTC-Software/Overview.html

Just like “Plants vs. Zombies” has become one of the most popular games, CRISPR/Cas system learned, borrowed and modified from the natural game “Bacteria vs. Phages” has been the hottest technology for genome editing. The CRISPR craze wept across scientific community last year. Synthetic Biology and iGEM were no exception. Twelve 2013 iGEM teams worked on CRISPR in their projects, with chassis varying from E. Coli, yeast, to plant and Mammalian. More than a dozen parts related to CRISPR/Cas were submitted last years. Some of them are in the DNA Kit of Parts for 2014 iGEM, shipped worldwide from the iGEM Headquarters. The sgRNA design tools are an important part for CRISPR/Cas technology and have attracted bioinformatician all over the world.

However, all available tools neglect the purpose of a given experiment, pay no attention to BioBricks standards, and do not support standard for synthetic biology data exchange (SBOL). Thus, a sgRNA design tool for genome editing in synthetic biology is desirable. In this project, we present CRISPR-X, a sgRNA design tool fully supporting SBOL and BioBricks standards with dynamic algorithm based on intent function, chassis, and newest experimental data. It adopts a C/S and B/S framework. The program on the server side is implemented with C and front-ends are implemented with Python (Browser ends) or JAVA ( Android ends, Desktop ends on Windows, Linux, and iOS), making it across all platforms.