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Team:Paris Bettencourt/Notebook/TMAU
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Paris Bettencourt 2014
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Notebook
Trimethylaminuria
June
Date 1
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Date 2
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July
July 9th
Miniprep, digestion and gel of pSB1C3 plasmid
Goal : extract PSB1C3 from E.coli and digest it. Result : failed (gel didn't work)=> restart on 10th July
Note. Close the bag with GeneJET Spin Columns tightly after each use!
20 kb, prewarm Elution Buffer to 70°C before applying to silica membrane.
2) Digestion of miniprep product with EcoR1 & Pst1 3) Gel migration => failure (pb with gel)
Date 2
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August
Date 1
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Date 2
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September
Date 1
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Date 2
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October
Date 1
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Date 2
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