Yesterday we transformed the chromoprotein and streaked M13, and we were expecting colorful plates. We have 10 plates full of colonies. They didn't show very clear colors (the pink and purple were easiest one to see; orange and lime were terrible because it's very similar to the nature color of our E.Coli and broth.) Our negative plate also had colonies. So we inoculate them today, along with RBS-Carb from glycerol stock.

Miniprep tomorrow. Hopefully the cells in liquid would show clearer colors and the broth would be colorful! We picked some sketchy-color-looking chromoprotein colonies. After that, we will do overnight digestion for the RBS-Carb to get the Card resistance gene out of the plasmid.

• Transform tcpD, toxT repoter vectors
• Inoculate chromoproteins, M13 parts
• Inoculate 3 tubes of i5.4.7 (RBS-Carb)

Description of image from today

Description of image from today