Team:RHIT/References

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References

• Molecular Cloning - A Laboratory Manual (1st-3rd editions), also knows as Maniatis, was consulted for all media recipes, as well as the competent cells protocol.

  
  

  Sambrook, J., and Russell, D. W. (2001). Molecular Cloning - A Laboratory Manual (3rd ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.





• The boil lysis miniprep protocol was acquired from the following reference:

  
  

  Boiling Lysis Mini Prep. Retrieved July 30, 2014, from http://www.oardc.ohio-state.edu/tomato/604.03/Module1/AlternativeMINIPREP.pdf





• Mini Pref and Gel Extraction protocols used were those received with Qiagen Kits.

  
  

  (2004). QIAprep Miniprep Handbook [Kit]. Germantown, MD.
  (2001). QIAquick Spin Handbook [Kit]. Germantown, MD.





• The Heat Shock Transformation protocol was obtained from Stanford University.

  
  

  Transformation Protocol Using Heat Shock.Retrieved June 12, 2014, from http://web.stanford.edu/~teruel1/Protocols/pdf/Transformation%20Protocol%20Using%20Heat%20Shock.pdf





• Agarose Gel Electrophoresis and DNA Digest protocols were obtained from Dr. Richard Anthony via personal communication.
  The ligation protocol was obtained from the New England BioLabs website.

  
  

  Quick Ligation Protocol (M2200). Retreived July 30, 2014, from http://www.neb.com/protocols/1/01/01/quick-ligation-protocol