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From 2014.igem.org

Contents 1 Friday 8th August 1.1 Lab work 1.1.1 D - Lemon Scent 1.1.1.1 PCR 1.1.1.2 Electrophoresis 1.1.1.3 Purification of the previous PCR 1.1.1.4 Stock of pcola plasmides 1.2 Human Pratices 1.2.1 Art & Design

Friday 8th August

Lab work

D - Lemon Scent

PCR

by Mélanie & Pierre We have done a PCR with pcola (geranyl synthase) and BBa_K517003:

• Green GotaqBuffer = 10µl
• MgCl2 = 4µl
• dNTP = 1µl
• Primer A = 1µM
• Primer B = 1µM
• DNA = 1µl
• Gotaq DNA polymerase = 0.25µl

Oligo used : iPS81bis AND iPS82 (pcola) iPS69 and iPS68bis (BBa_K517003)

PCR cycles:

• 95° 2min
• 95° 30sec
• 49-55° 30 sec
• 72° 2min
• 71° 5min

Electrophoresis

1. 10 µl of BBa_K517003
2. 10 µl of BBa_K517003
3. 10 µl of BBa_K517003
4. 10 µl of BBa_K517003
5. 10 µl of BBa_K517003
6. 10 µl of p. cola
7. 10 µl of p. cola
8. 10 µl of p. cola
9. 10 µl of p. cola
10. 10 µl of p. cola

Purification of the previous PCR

by Pierre

From the 5 tubes of p.Cola I cleaned up two tubes. From the 5 tubes of BBa_K517003 I cleaned up two tubes.

I did an electroforesis to check if the clening has worked. It did.

1. 1 µl of BBa_K517003
2. 1 µl of p. cola

Stock of pcola plasmides

using the DNA kit extraction to extract pcola ()

Human Pratices

Art & Design

by Terry

Sequel of yesterday molding attempt.

The mold is cut delicately and the test piece removed.

Then, the mold is placed back in the becher and filled up with regular agar ( 20mg/ml )

Result : FAILURE, the agar test piece has been ripped when unmolding. It was stuck to the mold.

But the mold is still intact ! I cleaned it up and slightly covered the inward surface with liquid soap. The mold is filled up again and conserved in the refrigerator for 2 hours.

Result : SUCCESS, I slightly remoistened the gel with water for an easier unmolding. The agar test piece is perfectly removed.