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Team:Evry/Notebook
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9mL MB
9mL M9
9mL 2xYT
9mL LB
M9 : the cells growth
2xYT : the cells growth
LB : the cells don't growth
Week 2: 28th July - 3th August
Friday, 1th August
Protocol for transformation
We transformed TOP 10 E. coli with plasmid of conjugation. This plasmid contains erythromycin like resistance cassette. I plated them on LB-Agar with erythomycin antibiotic.
Sunday, 3st August
Transformed cells
The cells transformed groth in the plate with erythromycin. I take one colonie in 3mL LB with erythromycin.
Tests culture pseudovibrio with medium
I take 1mL culture in M9 (aa and 3% NaCL)to 3 differents medium :9mL MB
9mL M9
9mL 2xYT
9mL LB
Week 1: 4thAugust - 10rd August
Monday, 4th August
Tests culture pseudovibrio with medium
MB : the cells growthM9 : the cells growth
2xYT : the cells growth
LB : the cells don't growth
Plasmid DNA puriication
A culture of E.coli transformed with plasmide of conjugation. A plasmid purification is realised with kit NucleoSpin.
After that 94,5ng.L-1 is purified.
Tuesday, 5th August
A culture of DH5a pir transformed with pNK2 (plasmid use to the transposas) in LB with ampicillin growth. After that the plasmid s purified.
