Team:Paris Saclay/Notebook
From 2014.igem.org
Notebook
We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.
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Planning
C - Salicylate Inducible Suppressing System
- Rehydration of BioBricks - 23th July
- Transformation of competent E.coli cells - 24th July
- Bacterial culture - 25th July
- Plasmid DNA Purification - 28th July
- Digestion to check & Electrophoresis - 28th July
- Main Digestion & Electrophoresis - 29th July
- DNA purification gel agarose - 30th July
- Ligation - 30th July
- Transformation in DH5 alpha - 31st July
- Bacterial Culture - 31st July
- Liquid Culture - 1st August
- Plasmid DNA Purification - 4th August
- Digestion to check & Electrophoresis - 4th August
- Final Stock - 4th August