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Team:ULB-Brussels/Project/Methods
From 2014.igem.org
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$
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First, birth and growing of bacteria populations (Centrifugation, Dilution). Secondly, insertion of the biobricks and plasmids chosen with E. Coli (Electroporation method, PCR Amplification, Electrophoresis). Just after this step, bacteria selection (in function of the quantities od oligopeptids or phoA+prolin) and inclusion of a toxin-antitoxin (TA: ccdB-ccdA) system in E. Coli. Then, addiction of fluorescent proteins (GFP, RFP) and determination of the quantities and the principal properties of our bacteria (including Emission Spectroscopy) and analize of their genetical sequences. Finally, conservation of bacteria populations that product the desired molecules in good quantities, at cold temperature in containers. | ||
- Université Libre de Bruxelles -
