Team:Oxford/protocols/Restriction Digest

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Restriction Digest

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  1. For a double digest, make sure that both enzymes are compatible and that a buffer exists in which both of them can work.

  2. Add 6 uL of water into an empty Eppendorf tube.

  3. Add 1uL of the buffer and 2 uL of the DNA or plasmid.

  4. Add 0.5uL of each enzyme solution. Make sure the restriction enzymes are kept on ice before use.

  5. Incubate for 1-2h at 37°C.