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Team:UCL/Diary/W2
From 2014.igem.org
Contents |
Week 2
Monday
Lab meeting
- in lab this week: Bez (lead), Edo, Sanjay, Dave, (YK & Sohaila supervising) NO 4 headed monster!
- monday restriction double digest to be done for: 5 lisbon plasmids (cut EcoRI+XbaI/uncut), 8 biobricks (cut EcoRI+PstI/uncut), following NEB restriction double digest protocol. LacI and RBS digest necessary for ligation. LacI digested with EcoRI SpeI, RBS with XbaI and Pstl. NEB restriction digest protocol for biobrick assembly. //YK add hyperlinks//
- tuesday: run gel, visualization for all 28 lanes of digest, 2 lanes of PCR from last week (friday/wednesday?) //YK// before darren's meeting (11am).
- tbd when: sohaila and YK go through the SOP for the assays with adam and ning. hopefully start the assembly of LacI + RBS ( + AzoR + Terminator) #3A
@YK need to email brian! what we need for lab space when we move
Edo
Cried when I saw that my free trial of snapgene expired. Back to pen and paper to do the calculations. Sent an email for sponsorship, finger crossed. Went to lab and prepared the digestion for gel visualisation and ligation of biobricks. Lunch with some good ideas for geeky gimmick, stay updated. Went to microfluidics lab and figured out how to fabricate some cool prototypes of devices.
Lewis M
Sanjay
Prepared restriction digests in the lab, pipetted so much I can barely type anymore. Watched Edo microwave his lunch. Our common room needs pillows so I can nap right now. Got some Bioprocess work done, and worked on the wiki. Good thing I can get drunk at the social tonight.
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
- Morning discussion about what we'll be doing this week at the lab meeting; primarily restriction digests to check what we have, so we're ready to ligate and assemble a BioBrick soon. With Lewis B, Soheila, Bez, Edo, Pamela, Dave, Sanjay, and Joy.
- In lab with Bez, Edo, Sanjay, and Dave. Prepared yet another fun restriction double digest for the 5 Lisbon plasmids, and 8 distribution BioBricks. Also digested the LacI //link// and RBS //link// in preparation for assembling/ligating our first BioBrick Device! (See Lab Notebook: 28/07/14. //link//)
- Had lunch in the quad with Bez and Edo, discussing a super-secret awesome HP idea that will help us win iGEM!
- Writing my iGEM diary whilst relaxing on the quad pillars/steps under the hot hot sun. Probably time to move on elsewhere and do something more productive! Impromptu frisbee in the quad begins.
- Running round getting printed copies of LiP design, cloning strategy, and lab freezer docs.
- Big lab meeting with most of the team to discuss points for tomorrow's general meeting.
- Finish the day with food and social at Huntley Bar!
- Note to self:
- Email Brian our lab requirements.
- Make RHnav scrolling.
Tanel
Adam
Kevin
Dave
Lewis B
Rob
Miriam
Here will go what I do from 28/07/2014 to 03/08/2014.
Alberto
Tuesday
Lewis M
Sanjay
Writing something here so Edo stops pestering me.
Maurice
Joy
Ning
Pamela
Bez
To do: Run gel, Follow up biotech sponsorship, Follow up debate, Update and tidy lab book
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Wednesday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Thursday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Friday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Weekend
