Contents 1 Week 2 1.1 Monday 1.1.1 Lab meeting 1.1.2 Edo 1.1.3 Lewis M 1.1.4 Sanjay 1.1.5 Maurice 1.1.6 Joy 1.1.7 Ning 1.1.8 Pamela 1.1.9 Bez 1.1.10 Daniel 1.1.11 Sohaila 1.1.12 Georgia 1.1.13 Yan-Kay 1.1.14 Tanel 1.1.15 Adam 1.1.16 Kevin 1.1.17 Dave 1.1.18 Lewis B 1.1.19 Rob 1.1.20 Myriam 1.1.21 Alberto 1.2 Tuesday 1.2.1 Lewis M 1.2.2 Sanjay 1.2.3 Maurice 1.2.4 Joy 1.2.5 Ning 1.2.6 Pamela 1.2.7 Bez 1.2.8 Daniel 1.2.9 Sohaila 1.2.10 Georgia 1.2.11 Yan-Kay 1.2.12 Tanel 1.3 Wednesday 1.3.1 Lewis M 1.3.2 Sanjay 1.3.3 Maurice 1.3.4 Joy 1.3.5 Ning 1.3.6 Pamela 1.3.7 Bez 1.3.8 Daniel 1.3.9 Sohaila 1.3.10 Georgia 1.3.11 Yan-Kay 1.3.12 Tanel 1.4 Thursday 1.4.1 Lewis M 1.4.2 Sanjay 1.4.3 Maurice 1.4.4 Joy 1.4.5 Ning 1.4.6 Pamela 1.4.7 Bez 1.4.8 Daniel 1.4.9 Sohaila 1.4.10 Georgia 1.4.11 Yan-Kay 1.4.12 Tanel 1.5 Friday 1.5.1 Lewis M 1.5.2 Sanjay 1.5.3 Maurice 1.5.4 Joy 1.5.5 Ning 1.5.6 Pamela 1.5.7 Bez 1.5.8 Daniel 1.5.9 Sohaila 1.5.10 Georgia 1.5.11 Yan-Kay 1.5.12 Tanel 1.6 Weekend

Week 2

Monday

Lab meeting

• in lab this week: Bez (lead), Edo, Sanjay, Dave, (YK & Sohaila supervising) NO 4 headed monster!
• monday restriction double digest to be done for: 5 lisbon plasmids (cut EcoRI+XbaI/uncut), 8 biobricks (cut EcoRI+PstI/uncut), following NEB restriction double digest protocol. LacI and RBS digest necessary for ligation. LacI digested with EcoRI SpeI, RBS with XbaI and Pstl. NEB restriction digest protocol for biobrick assembly. //YK add hyperlinks//
• tuesday: run gel, visualization for all 28 lanes of digest, 2 lanes of PCR from last week (friday/wednesday?) //YK// before darren's meeting (11am).
• tbd when: sohaila and YK go through the SOP for the assays with adam and ning. hopefully start the assembly of LacI + RBS ( + AzoR + Terminator) #3A

@YK need to email brian! what we need for lab space when we move

Edo

Cried when I saw that my free trial of snapgene expired. Back to pen and paper to do the calculations. Sent an email for sponsorship, finger crossed.

Lewis M

Sanjay

Prepared restriction digests in the lab, pipetted so much I can barely type anymore. Watched Edo microwave his lunch. Our common room needs pillows so I can nap right now. Got some Bioprocess work done, and worked on the wiki. Good thing I can get drunk at the social tonight.

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

1. Morning discussion about what we'll be doing this week at the lab meeting; primarily restriction digests to check what we have, so we're ready to ligate and assemble a BioBrick soon. With Lewis B, Soheila, Bez, Edo, Pamela, Dave, Sanjay, and Joy.
2. In lab with Bez, Edo, Sanjay, and Dave. Prepared yet another fun restriction double digest for the 5 Lisbon plasmids, and 8 distribution BioBricks. Also digested the LacI //link// and RBS //link// in preparation for assembling/ligating our first BioBrick Device! (See Lab Notebook: 28/07/14. //link//)
3. Had lunch in the quad with Bez and Edo, discussing a super-secret awesome HP idea that will help us win iGEM!
4. Writing my iGEM diary whilst relaxing on the quad pillars/steps under the hot hot sun. Probably time to move on elsewhere and do something more productive! Impromptu frisbee in the quad begins.
5. Note to self:
   • Email Brian our lab requirements.
   • Make RHnav scrolling.

Tanel

Adam

Kevin

Dave

Lewis B

Rob

Myriam

Alberto

Tuesday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

To do: Run gel Follow up biotech sponsorship Follow up debate

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Wednesday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Thursday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Friday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Weekend