Contents 1 Week 2 1.1 Monday 1.1.1 Lab meeting 1.1.2 Edo 1.1.3 Lewis M 1.1.4 Sanjay 1.1.5 Maurice 1.1.6 Joy 1.1.7 Ning 1.1.8 Pamela 1.1.9 Bez 1.1.10 Daniel 1.1.11 Sohaila 1.1.12 Georgia 1.1.13 Yan-Kay 1.1.14 Tanel 1.1.15 Adam 1.1.16 Kevin 1.1.17 Dave 1.1.18 Lewis B 1.1.19 Rob 1.1.20 Myriam 1.1.21 Alberto 1.2 Tuesday 1.2.1 Lewis M 1.2.2 Sanjay 1.2.3 Maurice 1.2.4 Joy 1.2.5 Ning 1.2.6 Pamela 1.2.7 Bez 1.2.8 Daniel 1.2.9 Sohaila 1.2.10 Georgia 1.2.11 Yan-Kay 1.2.12 Tanel 1.3 Wednesday 1.3.1 Lewis M 1.3.2 Sanjay 1.3.3 Maurice 1.3.4 Joy 1.3.5 Ning 1.3.6 Pamela 1.3.7 Bez 1.3.8 Daniel 1.3.9 Sohaila 1.3.10 Georgia 1.3.11 Yan-Kay 1.3.12 Tanel 1.4 Thursday 1.4.1 Lewis M 1.4.2 Sanjay 1.4.3 Maurice 1.4.4 Joy 1.4.5 Ning 1.4.6 Pamela 1.4.7 Bez 1.4.8 Daniel 1.4.9 Sohaila 1.4.10 Georgia 1.4.11 Yan-Kay 1.4.12 Tanel 1.5 Friday 1.5.1 Lewis M 1.5.2 Sanjay 1.5.3 Maurice 1.5.4 Joy 1.5.5 Ning 1.5.6 Pamela 1.5.7 Bez 1.5.8 Daniel 1.5.9 Sohaila 1.5.10 Georgia 1.5.11 Yan-Kay 1.5.12 Tanel 1.6 Weekend

Week 2

Monday

Lab meeting

• in lab this week: Bez (lead), Edo, Sanjay, Dave, (YK & Sohaila supervising) NO 4 headed monster!
• monday restriction double digest to be done for: 5 lisbon plasmids (cut EcoRI+XbaI/uncut), 8 biobricks (cut EcoRI+PstI/uncut), following NEB restriction double digest protocol. LacI and RBS digest necessary for ligation. LacI digested with EcoRI SpeI, RBS with XbaI and Pstl. NEB restriction digest protocol for biobrick assembly. //YK add hyperlinks//
• tuesday: run gel, visualization for all 28 lanes of digest, 2 lanes of PCR from last week (friday/wednesday?) //YK// before darren's meeting (11am).
• tbd when: sohaila and YK go through the SOP for the assays with adam and ning. hopefully start the assembly of LacI + RBS ( + AzoR + Terminator) #3A

@YK need to email brian! what we need for lab space when we move

Edo

Cried when I saw that my free trial of snapgene expired. Back to pen and paper to do the calculations. Sent an email for sponsorship, finger crossed.

Lewis M

Sanjay

Prepared restriction digests in the lab, pipetted so much I can barely type anymore. Watched Edo microwave his lunch. Our common room needs pillows so I can nap right now. Got some Bioprocess work done, and worked on the wiki. Good thing I can get drunk at the social tonight.

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

1. In lab with Bez, Edo, Sanjay, and Dave. Prepared yet another fun restriction double digest for the 5 Lisbon plasmids, and 8 distribution BioBricks. Also digested the LacI //link// and RBS //link// in preparation for assembling/ligating our first BioBrick Device! (See Lab Notebook: 28/07/14. //link//)
2. Had lunch in the quad with Bez and Edo, discussing a super-secret awesome HP idea that will help us win iGEM!
3. Writing my iGEM diary whilst relaxing on the quad pillars/steps under the hot hot sun.

Tanel

Adam

Kevin

Dave

Lewis B

Rob

Myriam

Alberto

Tuesday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Wednesday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Thursday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Friday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Weekend