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From 2014.igem.org

Contents 1 Friday 25th July 1.1 Lab work 1.1.1 C - Lemon scent 1.1.1.1 Results PCR targeting 1.1.1.2 Preparation and Transformation of competent cells 1.1.2 E - Salicylate Inducible Suppressing System 1.1.2.1 Bacterial culture: 1.2 Reunion

Friday 25th July

Lab work

C - Lemon scent

Results PCR targeting

by Romain

After the night, the electrophoresis revealed the oligonucleotides iPS70 and iPS71 prepared with the GoTaq enzyme.

We made a PCR Clean-up. Protocol

After that, we made another electrophoresis which revealed successfully the purification.

Preparation and Transformation of competent cells

E - Salicylate Inducible Suppressing System

Bacterial culture:

by Fabio

8 liquid cultures with 5ml of LB and 5µl of Amp, 4 with BBa_J61051 and 4 with BBa_K228001 (at 11am - 37°C - 150 rpm), all from the concentrated colony made the 24th July

Reunion

With Arnaud, Fabio, Sean, Terry, Alice, Solenne, Sylvie & Philippe

Members present:

• Instructors and advisors: Solenne.
• Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.

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