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From 2014.igem.org

Contents 1 Thursday 24th July 1.1 Lab work 1.1.1 C - Lemon scent 1.1.1.1 Results of the PCR Targeting 1.1.2 E - The Suppressing Salicylate System 1.1.2.1 Transformation of competent E.coli cells 1.1.3 ? - ????? 1.1.3.1 Gel electrophoresis of p cola

Thursday 24th July

Lab work

C - Lemon scent

Results of the PCR Targeting

by Romain

PCR targeting made the July 22nd.

Results:

• Nothing has grown on the control dishes.
• Failure, nothing has grown on the clone I dishes.
• Failure, other bacteria have grown on the clone II dishes.

We have to make another PCR.

E - The Suppressing Salicylate System

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α both BioBricks rehydrated yesterday (BBa_J61051 and BBa_K228001).

Samples:

1. BBa_J61051 Concentrated
2. BBa_J61051 Undiluted
3. BBa_K228001 Concentrated
4. BBa_K228001 Undiluted
5. DH5α Control

Protocol

Members present:

• Instructors and advisors: Alice and Solenne.
• Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.

? - ?????

Gel electrophoresis of p cola

by Sean

p cola used: sample left overnight yesterday resuspended in 30μl of H<2>O milliQ.

Protocol

Results

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