Team:Cambridge-JIC/Notebook/CL W4 Wednesday

From 2014.igem.org

Revision as of 11:29, 24 July 2014 by HuWilson (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Home Team Official Team Profile TimeLine Project Outreach Marchantia Informatics Parts Modeling Notebook Protocols Safety Dry Work Technology Attributions

Contents

Ginny's day

escaped catastrophe: primers made yesterday were pretty wrong...
remade new set_phew. MoClo_Biobrick compatible.
wetwork: co-cultured Marchantia spores with Agoro (RFP & GFP)
pursued secret side experiment___hold your breath

Trang-Anh's day

Got the codon counter to work (yay!) and ran the analysis on the monster. Obtained marchy codon table.
Looking at codon table: c preferred at the end of codons and c-g sites frequent, as in Arabidopsis.
Wrote up on the bioinformatics progress so far.

Will

Enhancer trap experimental design. Vector for random integration of minimal promoter into Marchy genome designed, and primers acquired. Jim gave me his vector for amplification of the minimal promoter (NB: it's an Arabidopsis promoter- although surely there must be some degree of homology between Eukaryotas, and *definitely* among plants). Ideally need Bernardo's vector as well. Primers arrive on Friday; will make vector then.

Hugh

More wiki work


Retrieved from "http://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_W4_Wednesday"