Team:Cambridge-JIC/Constructs/progress template

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Gal4 - Hap1GR - H2BmRFP

Design

Aim of the construct

Describe aims here

End goal plasmid

insert image here

Experimentation (up to date)

PCR

Attempt 1:

Start date/time:
End date/time:
UIDs of primers/plasmids used:
- A2, P12, P13
- B3, P14, P13
- A4, P12, P13
PCR Spreadsheet (on google drive)
Gel 27/07/2014

Gibson

Attempt 1:

Start date/time:
End date/time:
Comments:

E-Coli transformation

Attempt 1:

Start date/time:
End date/time:
Comments:

Agrobacteria transformation

Attempt 1:

Start date/time:
End date/time:
Induce comments:
Growth comments:
Electroporation/selection comments:

Spore preparation

Attempt 1:

Start date/time:
End date/time:
Comments:

Spore transformation

Attempt 1:

Start date/time:
End date/time:
Comments:

Evaluation

Start date/time:
End date/time:
Comments:

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