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From 2014.igem.org

Contents 1 Tuesday 22st July 1.1 Lab Work 1.1.1 1 - Samples for stock 1.1.2 2 - Electrophoresis 1.1.3 3 - Bacterial Culture 1.1.4 4 - Plasmid DNA extraction

Tuesday 22st July

Lab Work

1 - Samples for stock

by Fabio

We collected 1ml of each sample, added 240µl of glycerol (87%) and stored at -80°C.

• K1033902
• K1033905
• K1033910
• K1033913
• K1033922
• K1033925
• K1033927
• K1033929
• K103921
• K45017
• K731201
• K762100

from Liquide Bacterial Cultures transformed the 21st July

2 - Electrophoresis

by Arnaud (Process A), Fabio (gel), Marie and Romain (Process B)

We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel.

Process A

Process B

Results:

• A:
• B:
• C:

Protocol

3 - Bacterial Culture

by Fabio and Marie

One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony.

4 - Plasmid DNA extraction

by Sean and Terry

Plasmids used: cf part 1

Note: we accidentally used a buffer without 10% ethanol. After removal of the buffer, we repeated the step with a correct version. This may have affected the electrophoresis which followed.

Members there:

• Instructors and advisors: Solenne.
• Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.

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