Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP

From 2014.igem.org

Revision as of 18:23, 22 July 2014 by Salil (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Gal4 - Hap1GR - H2BmRFP>

Design

Aim of the construct

Describe aims here

End goal plasmid

insert image here

Experimentation (up to date)

PCR

Attempt 1:

Start date/time:
End date/time:
UIDs of primers/plasmids used:
A2, P12, P13
B3, P14, P13
A4, P12, P13
PCR Spreadsheet (on google drive)
Gel 27/07/2014

Gibson

Attempt 1:

Start date/time:
End date/time:
Comments:

E-Coli transformation

Attempt 1:

Start date/time:
End date/time:
Comments:

Agrobacteria transformation

Attempt 1:

Start date/time:
End date/time:
Induce comments:
Growth comments:
Electroporation/selection comments:

Spore preparation

Attempt 1:

Start date/time:
End date/time:
Comments:

Spore transformation

Attempt 1:

Start date/time:
End date/time:
Comments:

Evaluation

Start date/time:
End date/time:
Comments:

Retrieved from "http://2014.igem.org/Team:Cambridge-JIC/Constructs/gal4_hap1gr_H2RBmRFP"