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Team:Sumbawagen/Notebook/protocol7
From 2014.igem.org
Team:Sumbawagen/Team
From 2014.igem.org
Notebook – Protocol – 7. Ligation
1. Ligation reaction was as follow:
- Insert DNA, 2,5 ul*
- Plasmid DNA, 1.5 ul*
- Ligation kit (Takara), 5 ul Total volume, 10 ul
* final quantity insert DNA > plasmid DNA
2. Samples were incubated at 4 oC for overnight (inside a refrigerator).
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