Growing Liquid Cell Cultures

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1. Switch on the lamina flow hood and carry out steps 2 and 3 in the hood.
   
   
2. Pipette 5ml LB broth into a 15ml Falcon tube.
   
   
3. Inoculate the Falcon tube with strain being grown using an inoculation loop.
   
   
4. Grow at appropriate temperature (37°C for E. coli and 30°C for Pseudomonas) with shaking overnight.