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Team:Evry/Overview/Achievements/Projects
From 2014.igem.org
Projects Achievements
Chassis characterization
- - Genome Sequencing of Pseudovibrio denitrificans to collect informations defense mechanisms,
transposons putative insertion localisation and to possess a assembled genome for RNAseq. - - Growth conditions optimization
- - Antibiotic sensitivity tests shown a potential resistance to tetracycline and ampicilline
- - Electroporation Transformation protocol establishment that never been done before
New Vector
- - Transposon-based vector was created to overcome resistance mechanisms to DNA entrance
- - Proof of concept to use it in Pseudovibrio denitrificans was achieved
New or Optimized sensors Sensors
- - New PCB sensor based on bphR1 promoter and binding protein bphR2.
- - An optimized version of the Phenol sensors from Pekin iGEM 2013 team was constructed
- - RNA-seq data shall be used to find new promoters
