Team:ZJU-China/Safety

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Species Strain NIH Risk Group Disease risk to human?
E.Coli(K12) DH5-alpha 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) BL21(DE3) 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) DH10-beta 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) BW25113 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.

 

 

Our Safety Protocol

  1. Lab Safety

    Zhejiang University has set a strict protocol to ensure the safety of laboratory. Students are required to wear protective equipments including lab-gown, long pants, gloves, etc, while dealing with chemical and biological reagents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.

  2. Research Safety

    All students involved have been trained to operate equipments, reagents and strains in a safe way. We are not able to do ecperiments until we pass the bio-safety examination.

  3. Environment Safety

    All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used don't contain F plasmid, which guarantees that our genes would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.

  4. Reference

    http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia

    http://www.absa.org/riskgroups/

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