Team:Bordeaux/NotebookAugust5

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5th August

Molecular biology

  • Chewback: PCR step 1
    ELP-40 with oligo ELP-40 forward and reverse

  • Results of transformation :
    pSB1C3+RFP, 200μl BL21: 0 colonies
    pSB1C3+RFP, 20μl BL21: 0 colonies
    pSB1C3+RFP, 2μl BL21: 0 colonies
    ELP pUC57, 300μl BL21: 2 colonies
    ELP pUC57, 300μl DH5α: 2 colonies
    RLP pUC57, 300μl BL21: 97 colonies
    RLP pUC57, 300μl BL21: 162 colonies
    SLP pUC57, 300μl BL21: 9 colonies
    SLP pUC57, 300μl DH5α: 15 colonies
  • Try to make our biobricks ELP-40 and -60


Production

Production of ELP-40 and -60

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