Team:Virtus-Parva Mexico/Outcomes
From 2014.igem.org
Notebooks
Outcomes
The nanoparticlesWe ere capable of synthesizing magnetic nanoparticles with diameters of 22 nanometers. After coating them with Silicon dioxide their diameter was about 39 nanometers, these sizes were determined by Dynamic Light Scattering, a technique that shows the size distribution of the particles. The aminosilinization could not be confirmed by infrared spectra because the small amount of the amino groups in the sample. However the protein could be immobilized in the particle, showing us that the particles were correctly amino functionalized. |
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The Drill
Without a transmission electronic microscope it seemed a hard work to determine if the DNA and the protein had been attached to the nanoparticles, however we had another cheap yet powerful tool: Electrophoresis.
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