From 2014.igem.org

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4^th June

• ELP characterization
  • Test of polymere formation
    
    To get the thread of polymers we have tested in different polymers we observed the following results :
    
    - Sodium sulfate (Na2SO4) to 320g / L fiber => a fluffy appearance that does not take.
    - Diethyl ether (C2H5) 2O => no reaction because the drop is trapped we are finding that the solvent is immiscible.
    - Acetone (CH3COCH3) =>we observe the formation of a white ring.
    - Sodium chloride (NaCl 5M) hot => observation of a white cloud, which is due to a problem of cohesiveness.
    - Calcium chloride (CaCl2 15%) => we do a mixture of 1.25% alginate and ELP 40 (10 mg / ml) => fiber forming a perfect white.
    
    
    
    NB: test 0.75% of alginate PERFECT!
  
  
  • Test of transition
    
    After obtaining the fiber ELP 40 it is necessary to wash with hot water and extract; fiber may dissolve cold.
    
    - The control is transparent
    - The fiber changes color on contact at room Temperature, passing from white to transparent
    
    
    
    With the mixture Alginate ELP 40 + (10mg / L) we can observe the formation of the polymer and we can infer that :
    
    - In the presence of salt = the product disintegrates
    - In contact with water this contracts = warm
    
    
    
    
  • Wetspenning
  • Mechanical test :
    The traction machine can measure the degree of resistance to the breakage of any material.
    This experiment involves placing a small bar of the material to be studied between the jaws of a tensile testing machine that draws on the bar until it breaks. Elongation was recorded and the force applied, which is then converted into stress and strain.