From 2014.igem.org

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24^th July

• SLP gene synthesis
  
  
  
  PCR Parameter:
  92°C 1min
  12 cycles : 92°C 10s, 30 sec Gradient 67°C delta5°C : (12) (7) 67°C ; (34) (1) 65°C ; (45) (12) 69°C ; (56) (4) 66°C ; (67) (12) 69°C, 72°C 5s
  End at 4°C