From 2014.igem.org

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23^th July

• SLP gene synthesis
  
  
  
  PCR Parameter:
  98°c 30 sec
  35 cycles : 98°C 10 sec, 70°C 20 sec, 72°C 30 sec
  Final extension 5 min 72°C
  End at 4°C
  
  
  A second PCR was performed on the tube ABC° with the same parameter as before.