From 2014.igem.org

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15^th September

• SDS-PAGE
  Resuspension of 1-2mg of lyophilized ELP in cold PBS
  
  We make a range:
  
  

  ELP	7μl	13μl
  PBS	6μl	0μl
  Sample Buffer 2X	7μl	7μl

  
  
  Heat 3min at 85°C
  => Coloration with Coomassie Brilliant blue and decoloration