Team:Bordeaux/NotebookAugust26

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26th August

Molecular biology

  • Results of transformation
    pSB6A1 : 193 CFU
    ELP-ELP 1: 1776 CFU
    ELP-ELP 2: 1104 CFU
    ELP-SLP: 2016 CFU
    ELP-SLP: 2400 CFU
    ELP-RLP: 1876 CFU
    ELP-RLP: 2368 CFU
    SLP-SLP: 93 CFU
    SLP-SLP: 91 CFU
    SLP-RLP: 10 CFU
    SLP-RLP: 248 CFU
    RLP-RLP: 1872 CFU
    RLP-RLP: 1424 CFU

  • Digestion pSB1C3
    Buffer (cutsmart) 5μl
    Xba1 0,5μl
    Spe1 0,5μl
    DNA 10μl
    H2O qsp 50μl

    => 1h at 37°C and gel 1% (30min, 100V)

  • Miniprep pSB1C3
    Sample 1: 224,2ng/μl
    Sample 2: 313,4ng/μl<
    Sample 3: 269,9ng/μl

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