From 2014.igem.org

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26^th August

• pSB6A1 : 193 CFU
  ELP-ELP 1: 1776 CFU
  ELP-ELP 2: 1104 CFU
  ELP-SLP: 2016 CFU
  ELP-SLP: 2400 CFU
  ELP-RLP: 1876 CFU
  ELP-RLP: 2368 CFU
  SLP-SLP: 93 CFU
  SLP-SLP: 91 CFU
  SLP-RLP: 10 CFU
  SLP-RLP: 248 CFU
  RLP-RLP: 1872 CFU
  RLP-RLP: 1424 CFU
  


• Digestion pSB1C3

  Buffer (cutsmart)	5μl
  Xba1	0,5μl
  Spe1	0,5μl
  DNA	10μl
  H2O	qsp 50μl

  
  => 1h at 37°C and gel 1% (30min, 100V)


• Miniprep pSB1C3
  Sample 1: 224,2ng/μl
  Sample 2: 313,4ng/μl<
  Sample 3: 269,9ng/μl