"

From 2014.igem.org

15^th July

• Gene SLP synthesis
  
  PCR step 3 : Vtot = 50µL
  
  10µL phusion buffer 5X
  1µL dNTP 10mM
  0,5µL phusion polymérase
  2,5µL DMSO
  2,5µL Primer 1 à 10mM
  2,5µL Primer 8 à 10mM
  10µL 3° ou 3°°
  21µL H2O
  
  PCR Parameter:
  98°C 5 min
  35 cycles : 98°C 10s, 69°C 20s, 72°C 1min
  Final extension 72°C 10min
  End at 4°C
  
  
  
  
  
  

Please look our planning of production and purification