"
Team:UFMG Brazil/Project/Notebook
From 2014.igem.org
| February | |
|---|---|
| 1º week | *First meeting with the team |
| 2º week | |
| 3º week | |
| 4º week | |
| 5º week | |
| March | |
| 1º week | |
| 2º week | |
| 3º week | *Possibilities of themes: → Metabolic syndrome, → Promoter sensitive to sound → Use of yeast *Doodle *Description *Trying to get support from Pro-reitoria→ Professor. Adelina *Sponsorship |
| 4º week | *Defined theme: →Use of yeast for detection and treatment of colon cancer |
| 5º week | *Vakinha (Crowd funding) |
| April | |
| 1º week | *Defined theme: →Use of yeast for detection and treatment of colon cancer |
| 2º week | *Cancer of colon *Probiotic: UFMG 905 *Biomarkers: sensors *Human practices (Meagan) → Difficulties to do Science in Brazil →Documentary → Buttons "I'm from Latin America" → Lectures |
| 3º week | *Identification system Colon cancer (Carlos) →Quantify the DNA present in the feces of mice with cancer (Laila) →Kit for DNA extraction from faeces (Rodrigo); →Disclosure TV radio and medias →Refresh the iGEM Facebook page →Search for new options field of non-specific DNA binding |
| 4º week | *Long strand DNA →Extracting DNA from faeces of mice →System *Chimera proteins: look at other teams * Importance of attendance at team meetings * Romario: looking press office *Oncologists →Situation of colon cancer →Diagnosis *CCSP-2 (Oncogenes 2005) → Von Willebrand →Search for markers →DNA (Carlos) →Carbohydrates (Laila / Isadora / Lucas) →CCSP-2 (Rodrigo and Michele) →Metabolites (Marcelo and Dani) *Need to find for sponsorship (Ciça and Chris) *Idea to elaborate questionnaire to other Latin American iGEM teams (Thiago and Veronica) |
| 5º week | *TALE: Specific Sequence. DNA binding *mCherry split: NEW !!! *Can we use FRET techniques? *mCherry modeling *Microsatellite *Synthesis of template DNA repeat *Ca-binding proteins overexpressed ++ *Biosafety *CCSP-2: two domains von wilebrand *Workers yeasts: central information (more than one signal "warning" the same cell) |
| May | |
| 1º week | *Division of the responsibilities: →Draw the biobricks (Carlos) →Collecting mice faeces (Laila) →Protocol for DNA extraction from faeces and quantification (Rodrigo and Michele) →Human Practices (Thiago Mafra) →Cancer in the past IGEM (Marcelo and Dani Chame) →Video animation of IGEM UFMG BRAZIL (Lucas and Isadora) →Sponsors (Cecilia, Chris, James and Veronica Bruno) →Translate questionnaire to Spanish (Dani Chame) |
| 2º week | *Conversation with Paulo Sérgio Lacerda Beirão →Legislation of the country *Videos with testimonials about the science in the country of researchers |
| 3º week | *Metabolic Syndrome - network of diseases *Biomarkers choice → limit →Complexity →Physiology →Stability *Lenght DNA→ eletricity (3’ 5’) *Accumulation of signal *Transfer of fluorescence *DNA bound proteins: proteins crawling in the DNA *Noise/signal ratio: important for qualitative diagnosis *Stool: noise *Proximity can allow interaction with domains that will activate an enzyme that transform a smelly (banana?) substract OR *Smell constitutive? *Yeast die if it dos not meet signal. *Difference between short and long DNA *NEW idea: sound detection. *mCherry |
| 4º week | *Protocol for feces *Modeling (Gastone) →Probability of false positives *Yeast plasmids design *Paratope Fab / Histone *Validation in E.coli: pass after apra yeast synthesize: →TALE →mCherry →Long repetitive DNA →Short repetitive DNA |
| 5º week | |
| June | |
| 1º week | *Workshop Biosynth *How to earn money? →Brazilian companies? →INCT →Proreitoria →Mario Penna *Latin America Fan Page |
| 2º week | *RFC10 - Biobrick standard *Bsmb1 → TALE *Software Assembly? *Wiki *Wet lab - gene synthesis *How to earn money? |
| 3º week | *Discount in the synthesis *Enzymes: XbaI, SpeI, EcoR1, Pst1, AgeI, NgomIV, BsmbI →Phoneutria? *Jacques Nicoli→ Yeast *Biosafety (term 23/06) *Donation Fiocruz |
| 4º week | *New England Enzyme *Texts Wiki →Project →Team →Human practices →Vakinha (Crowd funding) |
| 5º week | |
| July | |
| 1º week | * Experimental tests * shopping * Restriction enzymes * length of 110 nucleotides linker → → try a smaller *experimental →Biobrick assemble the plasmid →Sequencing →Express and perform the tests in vitro. →Write protocols for wiki *Modeling mathematics *Wiki: →Assemble layout and content. *Human Practices →Questionnaires →Sponsorships →Questions - students ufmg schools. *Bioethics |
| 2º week | *Price for synthesizing biobrick →Request assistance pro-reitoria *Modeling →test of interaction assemblies TALE + + Linker Hmcherry1 and 2 *Making a letter for sponsorship |
| 3º week | *Deadlines * Fundraising Ideas * Human Practices → Send e-mails with the questionnaires * Modeling of proteins *Extraction of faeces |
| 4º week | |
| 5º week | |
| August | |
| 1º week | * Arrival of restriction enzyme → Work *Texts for wiki *inscription IGEM |
| 2º week | *Experiments |
| 3º week | *Sponsors |
| 4º week | *Experiments * Human Practices →Results of the questionnaires * Modeling of proteins |
| 5º week | |
| September | |
| 1º week | *Meeting with Dr.Clélio Campolina, National minister of Science and Technology *Wiki |
| 2º week | *Human Practices: low adhesion *Fetch data from investment in research *Participation in Latin America over the years in the iGem *Plot: X number of contacts feedback *Lack of appreciation of Science *Popular search |
| 3º week | |
| 4º week | *Experiments *Project Summary *Meeting with Dr. Evaldo Vilela, FAPEMIG director. |
| 5º week | |
| October | |
| 1º week | *Finish the Wiki Modeling *Experimental *Human practices. →Questionnaire in the 7 square |
| 2º week | *Lecture to high school, Technical school and Graduation, at CEFET. * Survey: The Importance of Science * Concentrations DNA * Sponsors * Deadlines |
| 3º week | * Experiments →Results * Human Practices →Results *Modeling of proteins →Results * Modeling mathematics →Results * Sponsors →Tshirt →Who helped? |
| 4º week | |
| 5º week | |
| November | |
| World Championship Jamboree. |
