Team:Bordeaux/NotebookAugust20
From 2014.igem.org
20th August
Molecular biology
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Test of pSB1C3 miniprep
- Migration on 1% agarose gel (100V, 30min)
- Making daily competent cells (BL21) and transformation with pSB1C3 (spread on LB+chlo plates)
Production
Problem with preculture => ampicillin
Purification
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End of the purification for ELP-40 and -60