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Team:UC Davis/Protein Engineering Design
From 2014.igem.org
Why Aldehyde Dehydrogenases?
The aldehyde dehydrogenase family of enzymes (EC: 1.2.1.3, 1.2.1.5) was selected for use with our electrochemical biosensor. This family of enzymes catalyze the reaction of aliphatic, straight chain aldehydes and the oxidized form of beta-nicotinamide adenine dinucleotide (NAD+) to produce the corresponding carboxylic acid and the reduced form of beta-nicotinamide adenine dinucleotide (NADH).
The aldehyde dehydrogenase enzyme family is perfect our engineering and electrochemical applications:
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This enzyme uses NAD+ as a coenzyme and produces NADH in a 1:1 molar ratio with the amount of aldehyde catalyzed. The concentration of NADH can be readily measured with a spectrophotometer reading absorbance at 340nm, allowing us to easily measure the rate of the reaction catalyzed by an aldehyde dehydrogenase enzyme.
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The active site of aldehyde dehydrogenase is in the center of a long tunnel, where NAD+ enters from one side and the aldehyde substrate enters from the other side. This tunnel (highlighted in orange) gives us a large amount of flexibility in engineering amino acid residues which will alter the catalytic efficiency of this enzyme toward certain aldehyde species.
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We identified several commercial electrodes which oxidize NADH back to NAD+ and produce a current. This will allow us to measure the amount of NADH produced over time using an electrochemical approach.
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Preferred: Crystal structure available on the Protein Data Bank (PDB)
With aldehyde dehydrogenases in mind, we used two approaches to identify enzymes with the desired specificities we would use in our biosensor: bioprospecting and engineering.
Approach 1: Bioprospecting
We used several online databases to search for aldehyde dehydrogenases with unique specificity profiles. Keeping the context of the aldehyde dehydrogenase within our electrochemical sensor in mind, we focused exclusively on aldehyde dehydrogenases which used NAD(H) as a coenzyme (EC: 1.2.1.3) as opposed to aldehyde dehydrogenases which used NADP(H) (EC: 1.2.1.5). However, we did identify several aldehyde dehydrogenases which could use either coenzyme. BRENDA was our main resource for identifying aldehyde dehydrogenases which catalyzed the oxidation reaction on specific substrates. The specificity data provided within the literature cited for each entry on BRENDA helped guide our decisions for the set of enzymes we chose to characterize.
Selection Criteria:
DNA sequence is readily available on Genbank or UniProtKB
Aldehyde dehydrogenase possesses unique specificity for one or more of the aldehyde species known to occur in olive oil
Aldehyde dehydrogenase uses NAD+ as a coenzyme for the dehydrogenase reaction
Aldehyde dehydrogenase is documented to express in Escherichia coli and is easily purified in high yield (>1 mg/mL)
| Source | UniProtKB ID | EC Number | Crystal Structure Available? |
|---|---|---|---|
| Escherichia coli (Strain K12) | P23883 | 1.2.1.5 | No |
| Rhodococcus erythropolis | Q4F895 | 1.2.1.3 | No |
| Rattus norvegicus (Rat) | P11883 | 1.2.1.5 | 1AD3 |
| Human (ALDH3A1) | P30838 | 1.2.1.5 | 3SZA |
| Human (ALDH2) | P05091 | 1.2.1.3 | 1O01 |
Approach 2: Engineering
We chose the aldehyde dehydrogenase from Escherichia coli as our template for engineering additional specificity profiles outside of the ones we identified in the literature. While there was not a crystal structure available for E. coli aldehyde dehydrogenase, we decided it would be the best template for engineering since it expressed the best (>3 mg protein per 25mL culture) out of all the aldehyde dehydrogenase we investigated. Thus, we generated a homology model of E. coli aldehyde dehydrogenase and used the interactive protein folding design software Foldit to design mutants. Investigation of the homology model and crystal structures of other aldehyde dehydrogenases revealed that substrates must travel through a tunnel composed of hydrophobic residues to reach the catalytic center of the enzyme. Taking this into consideration, our design strategy was to alter the residues in the substrate tunnel in an attempt to change the enzyme's catalytic efficiency towards certain substrates.
We used four strategies for designing our mutants:
Introducing hydrophilic residues in the substrate tunnel to discourage catalysis of more hydrophobic aldehydes
Increasing solvent exposure to the catalytic center by introducing smaller residues in the tunnel
Increasing packing around catalytic center with larger hydrophobic residues
Introducing positive/negative charges in the substrate tunnel
We designed a total of 20 mutants:
| Mutations | Description |
|---|---|
| D119I;F169A;Y296A | Increase solvent exposure to active site, open enterance to tunnel |
| D119L;Y296F;N455A | Increase hydrophobicity at far end of tunnel |
| D456L;D458L | Change far pocket polar residues to posess more nonpolar characteristics |
| F169A | Open up active site adjacent to catalytic residues w/ hydrophobic |
| F169D | Introduce negative charge adjactent to active site |
| F169H;F463H | Introduce mild positive charge adjactent to active site |
| F169L;W176L;F463L | Open up active site adjacent to catalytic residues w/ hydrophobic |
| F169N;L173N;W176Q;I303Q;F463S | Super solvent exposure to active site |
| F463W | Better packing around catalytic residues |
| G457E | G457E occurs in PDB: 1O01, may be natural source of substrate specificity |
| I303M | Better packing around catalytic residues |
| I303Q | Introduce hydrophilic character to active site adjactent to catalytic residues |
| I303S;F463S | Introduce hydrophilic character to active site adjactent to catalytic residues |
| K475M | Remove negative charge from tunnel |
| L172Y;Y296W;W176V | Decrease size of hydrophic tunnel region |
| V301D | Introduce negative charge adjactent to active site |
| V301H | Introduce mild positive charge adjactent to active site |
| W176Q | Introduce hydrophilic character to active site adjactent to catalytic residues |
| W176V | Increase size of hydrophobic pocket at the alkyl end of 2-butenal |
| Y296L | Created salt bridge at entrance of tunnel |
