Team:Bordeaux/NotebookAugust5

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4^th June

Molecular biology

Chewback: PCR step 1
ELP-40 with oligo ELP-40 forward and reverse

Results of transformation :
pSB1C3+RFP, 200μl BL21: 0 colonies
pSB1C3+RFP, 20μl BL21: 0 colonies
pSB1C3+RFP, 2μl BL21: 0 colonies
ELP pUC57, 300μl BL21: 2 colonies
ELP pUC57, 300μl DH5α: 2 colonies
RLP pUC57, 300μl BL21: 97 colonies
RLP pUC57, 300μl BL21: 162 colonies
SLP pUC57, 300μl BL21: 9 colonies
SLP pUC57, 300μl DH5α: 15 colonies
Try to make our biobricks ELP-40 and -60

Production

Production of ELP-40 and -60

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