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Team:UIUC Illinois/Protocols
From 2014.igem.org
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Gel Electrophoresis
Summarize Protocol Here
PCR Set-Up
Summary Paragraph
Bacterial Transformation
Summarize Protocol Here
Restriction Endonuclease Digest
Summary Paragraph
CaCl2 Competent Cell Protocol
Summarize Protocol Here
Genomic DNA Purification
Summary Paragraph
Resting Cell Assay
Summarize Protocol Here
Colony PCR
Summary Paragraph
Lactobacillus Transformation
- Inoculate 100mL MRS with 1mL of an overnight culture and incubate at 37C until it reaches an OD600 = 0.5 – 0.8.
a. Pre-warm the MRS for faster growth b. Take 3.5-5.5 hours to get to 0.5 c. All liquid cultures can be done outside the anaerobic chamber; however, do not use a shaker
The Institute for Genomic Biology
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